Abstract: Rural road networks often lack the physical and digital infrastructure required for the safe and reliable operation of automated vehicles (AVs). Inadequate or poorly maintained lane markings, variable road surface conditions, limited GPS reliability, and uneven 5G network coverage pose challenges for automated driving systems. While such conditions may be manageable for human drivers, their readiness for AV operation has not been systematically evaluated, resulting in limited focus on rural AV infrastructure studies. This project developed a framework to assess rural road infrastructure readiness for AV deployment. The framework considers physical and digital infrastructure elements, including lane markings, GPS accuracy, and 5G coverage. To implement it, a software tool was developed to process, quantify, and visualize offline data collected using cameras, GPS, and 5G modules deployed on AV platform vehicles in selected rural areas of North Carolina. Experimental evaluation demonstrated that the framework and tool enable systematic identification and quantification of infrastructure limitations affecting AV deployment.